Two interconvertible forms of tryptophanyl sRNA in E. coli.

8 Felsenfeld, G., and S. Huang, Biochim. Biophys. Acta, 51, 19 (1961). 9 Lyons, J. W., and L. Kotin, J. Am. Chem. Soc., 86, 3634 (1964). 10 Boedtker, H., J. Mol. Biol., 2, 171 (1960). 11 Monier, R., and M. Grunberg-Manago, in Acides Ribonucleiques et Polyphosphates, Colloques Internationaux de Centre National du la Recherche Scientifique no. 106 (Paris, 1962), p. 163. 12 Giacomoni, D., and S. Spiegelman, Science, 138, 1328 (1962). 13Mahler, H. R., G. Dutton, and H. Mehrotra, Biochim. Biophys. Acta, 68, 199 (1963). 14 Nishimura, S., and G. D. Novelli, Biochem. Biophys. Res. Commun., 11, 161 (1963). 11 Nishimura, S., and G. D. Novelli, Biochim. Biophys. Acta, 80, 574 (1964). 16 Penniston, J. T., and P. Doty, Biopolymers, 1, 145 (1963). 17 Allende, J. E., C. C. Allende, M. Gatica, and M. Matamala, Biochem. Biophys. Res. Commun., 16, 342 (1964). 18 Norris, A., and P. Berg, these PROCEEDINGS, 52, 330 (1964). 1I Lagerkvist, U., and J. Waldenstrom, J. Biol. Chem., 240, Pc 2264 (1965). 20 Allende, J. E., G. Mora, M. Gatica, and C. C. Allende, J. Biol. Chem., 240, Pc 3229 (1965). 21 Cf. P. Berg, Ann. Rev. Biochem., 30, 293 (1961). 22 Holley, R. W., Biochem. Biophys. Res. Commun., 10, 186 (1963). 23 Lindahl, T., and J. R. Fresco, in Methods in Nucleic Acids, ed. L. Grossman and K. Moldave (New York: Academic Press, 1966), vol. 1, in press. 24 Lindahl, T., D. Henley, and J. R. Fresco, J. Am. Chem. Soc., 87, 4961 (1965). 26 Tissibres, A., J. Mol. Biol., 1, 365 (1959). 26 Loftfield, R. B., and E. A. Eigner, Acta Chem. Scand., 17, S117, (1963). 27 Holley, R. W., J. Apgar, G. A. Everett, J. T. Madison, S. H. Merrill, and A. Zamir, in Cold Spring Harbor Symposia on Quantitative Biology, vol. 28 (1963), p. 117. 28 Apgar, J., R. W. Holley, and S. H. Merrill, J. Biol. Chem., 237, 796 (1962). 29 M6ller, W., and H. Boedtker, in Acides Ribonucleiques et Polyphosphates, Colloques Internationaux de Centre National du la Recherche Scientifique no. 106 (Paris, 1962), p. 99. 30 The presence of several sRNA components for both leucine and arginine in yeast has been described earlier. Thiebe and Zachau observed two leucyland three arginyl-sRNA's after countercurrent distribution in a solvent system different from that used here [Thiebe, R., and H. G. Zachau, Biochim. Biophys. Acta, 103, 568 (1965)]. Cherayil and Bock found three distinct peaks for leucyl acceptor activity by chromatography on DEAE columns in the presence of urea [Cherayil, J. D., and R. M. Bock, Biochemistry, 4, 1174 (1965)]. Sequence analysis on C14-leucyloligonucleotides obtained after enzymatic digestion of aminoacyl-sRNA gave evidence for the presence of three (or more) different leucyl sRNA's (Herbert, E., personal communication).